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    Interface of Phospholipid Polymer Grafting Layers to Analyze Functions of Immobilized Oligopeptides Involved in Cell Adhesion

    Year: 2020

    Journal: ACS Biomater. Sci. Eng., Volume 6, JUL, page 3984–3993

    Authors: Ishihara, Kazuhiko; Ito, Misaki; Fukazawa, Kyoko; Inoue, Yuuki

    Keywords: cell adhesion; poly(2-methacryloyloxyethyl phosphorylcholine); surface-initiated graft polymerization; cell adhesive molecules; movability of polymer chains

    The aim of this study was to design a material surface for use in the analysis of the behavior of biomolecules at the interface of direct cell contact. A superhydrophilic surface was prepared with poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC), which was grafted onto a substrate with controlled polymer chain density. An arginine-glycine-aspartic acid (RGD) peptide was immobilized at the surface of the polymer graft surface (PMPC-RGD surface). Initial adhesion of the cells to this substrate was observed. The PMPC-RGD surface could enable cell adhesion only through RGD peptide-integrin interactions. The density and movability of the RGD peptide at the terminal of the graft PMPC chain and the orientation of the RGD peptide affected the density of adherent cells. Thus, the PMPC graft surface may be a good candidate for a new platform with the ability to immobilize biomolecules to a defined position and enable accurate analysis of their effects on cells.
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