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    Investigation on the Interactions between Self-Assembled beta-Sheet Peptide Nanofibers and Model Cell Membranes

    Year: 2020

    Journal: Int. J. Mol. Sci., Volume 21, DEC

    Authors: Waku, T; Kasai, A; Kobori, A; Tanaka, N

    Organizations: Japan Society for the Promotion of Science (JSPS) KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [19K12800]

    Keywords: nanofiber; DPPC Langmuir membrane; π – A isotherm; drug delivery system

    Self-assembled peptide nanofibers (NFs) obtained from beta-sheet peptides conjugated with drugs, including antigenic peptides, have recently attracted significant attention. However, extensive studies on the interactions of beta-sheet peptide NFs with model cell membranes have not been reported. In this study, we investigated the interactions between three types of NFs, composed of PEG-peptide conjugates with different ethylene glycol (EG) lengths (6-, 12- and 24-mer), and dipalmitoylphosphatidylcholine (DPPC) Langmuir membranes. When increasing the EG chain length, those interactions significantly decreased considering measurements in the presence of the NFs of: (i) changes in surface pressure of the DPPC Langmuir monolayers and (ii) surface pressure-area (pi-A) compression isotherms of DPPC. Because the observed trend was similar to the EG length dependency with regard to cellular association and cytotoxicity of the NFs that was reported previously, the interaction of NFs with phospholipid membranes represented a crucial factor to determine the cellular association and toxicity of the NFs. In contrast to NFs, no changes were observed with varying EG chain length on the interaction of the building block peptide with the DPPC membrane. The results obtained herein can provide a design guideline on the formulation of beta-sheet peptide NFs, which may broaden its potential.
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