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    Label- and enzyme-free detection of glucose by boronic acid-coupled poly(styrene-b-acrylic acid) at liquid crystal/aqueous interfaces

    Year: 2018

    Journal: Anal. Chim. Acta, Volume 1032, NOV 22, page 122–129

    Authors: Munir, Sundas; Park, Soo-Young

    Organizations: Kyungpook National University Bokhyeon Research Fund, 2016

    Keywords: Non-enzymatic biosensor; Liquid crystal; Polyelectrolyte brush; 3-Aminophenyl boronic acid; Glucose

    A glucose biosensor was developed based on a 4-cyano-4'-pentylbiphenyl (5CB)-filled transmission electron microscopy (TEM) grid cell by coating poly (styrene-b-acrylic acid) (PS-b-PAA) at the 5CB/aqueous interface and immobilising 3-aminophenyl boronic acid (APBA) on the PAA block chains by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide coupling. Binding events between APBA and glucose were translated into homeotropic-to-planar configurational changes of 5CB, which were observed by polarised optical microscopy (POM) under crossed polarisers. This liquid-crystal-based glucose biosensor exhibited high sensitivity (limit of detection: 0.1 mM), even in complex serum and urine samples, high selectivity (against cholesterol, haemoglobin, and urea), and good stability for 40 d. This new and sensitive glucose biosensor has the advantages of low production cost, a simple immobilisation technique, and easy detection with POM, and may be useful for pre-screening glucose levels in human samples (serum and urine). (C) 2018 Elsevier B.V. All rights reserved.
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