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    Label-Free Detection of Staphylococcus aureus Based on Bacteria-Imprinted Polymer and Turn-on Fluorescence Probes

    Year: 2021

    Journal: ACS Appl. Bio Mater., Volume 4, JAN 18, page 420–427

    Authors: Guo, Yuanyuan; Li, Juan; Song, Xiuling; Xu, Kun; Wang, Juan; Zhao, Chao

    Organizations: National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [82073603, 82073557]; Jilin Province Science and Technology Development Plan Item [20200602010ZP, 20200403035SF]

    Keywords: food safety; label-free detection; Staphylococcus aureus; bacteria imprinted; turn-on fluorescence

    The effective identification and quantitative determination of Staphylococcus aureus is a major public health concern. Here, an innovative strategy that combines a bacteria-imprinted polydimethylsiloxane film for bacterial recognition and fluorescence resonance energy transfer platform for turn-on fluorescence sensing is demonstrated. The bacteria-imprinted polydimethylsiloxane film was facilely fabricated to generate corresponding specific sites on the polydimethylsiloxane surface via stamp imprinting using Staphylococcus aureus as template followed by modification with 1H,1H,2H,2H-perfluorooctyltriethoxysilane. The fluorescence resonance energy transfer platform was developed through electrostatic interaction between citrate-functional copper clusters and dopamine-stabilized gold nanoparticles. When the Staphylococcus aureus are present, the 1H,1H,2H,2H-perfluorooctyltriethoxysilane-modified bacteria-imprinted polydimethylsiloxane film can precisely capture the target; subsequently, the negatively charged bacteria compete with citrate-functional copper dusters and bind to dopamine-stabilized gold nanoparticles, leading to the fluorescence recovery of citrate-functional copper dusters. The entire detection process was achieved within 135 min, showing a wide linear calibration response from 10 to 1 x 10(7) cfu mL(-)(1 )with a low detection limit of 11.12 cfu mL(-1). Furthermore, the recoveries from spiked samples were from 97.7 to 101.90% with relative standard derivations lower than 10%. The established label-free assay of measuring Staphylococcus aureus is rapid, sensitive, specific, and efficient.
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