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    Linoleic and linolenic acid hydroperoxides interact differentially with biomimetic plant membranes in a lipid specific manner

    Year: 2019

    Journal: Colloid Surf. B-Biointerfaces, Volume 175, MAR 1, page 384–391

    Authors: Deleu, Magali; Deboever, Estelle; Nasir, Mehmet Nail; Crowet, Jean-Marc; Dauchez, Manuel; Ongena, Marc; Jijakli, Haissam; Fauconnier, Marie-Laure; Lins, Laurence

    Organizations: Fonds pour la formation a la Recherche dans l'Industrie et dans l'Agriculture (FRIA) grant from the F.R.S.-F.N.R.S (Fonds National de la Recherche Scientifique, Belgium)Fonds de la Recherche Scientifique - FNRS [5100617F]; University of Liege (ARC-FIELD project) [13/17-10]; F.R.S.-FNRSFonds de la Recherche Scientifique - FNRS [2.5020.11, PDR T.1003.14, CDR J.0086.18, CDR J.0114.18]; HPC-Regional Center ROMEO from the University of Reims Champagne-Ardenne (France); Multiscale Molecular Modeling Platform from the University of Reims Champagne-Ardenne (France)

    Keywords: Oxylipins; Plant membrane; Molecular interactions; Lipid specificity

    Linoleic and linolenic acid hydroperoxides (HPOs) constitute key intermediate oxylipins playing an important role as signaling molecules during plant defense processes in response to biotic or abiotic stress. They have also been demonstrated in vitro as antimicrobial agents against plant fungi and bacteria. To reach the phytopathogens in vivo, the HPOs biosynthesized in the plant cells must cross the plant plasma membrane (PPM) where they can also interact with plasma membrane lipids and have an effect on their organization. In the present study, we have investigated the interaction properties of HPOs with PPM at a molecular level using biophysical tools combining in vitro and in silico approaches and using plant biomimetic lipid systems. Our results have shown that HPOs are able to interact with PPM lipids and perturb their lateral organization. Glucosylceramide (GluCer) is a privileged partner, sitosterol lessens their binding and the presence of both GluCer and sitosterol further reduces their interaction. Hydrophobic effect and polar interactions are involved in the binding. The chemical structure of HPOs influences their affinity for PPM lipids. The presence of three double bonds in the HPO molecule gives rise to a higher affinity comparatively to two double bonds, which can be explained by their differential interaction with the lipid polar headgroups.
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