Contact
    Start Publications Modulation of membrane physical properties by natural ...
    ←  Publication Finder
    KSV NIMA

    Modulation of membrane physical properties by natural insecticidal ketones

    Year: 2021

    Journal: Biophys. Chem., Volume 269, FEB

    Authors: Miguel, V; Sanchez-Borzone, M. E.; Mariani, M. E.; Garcia, D. A.

    Organizations: SECyT-Universidad Nacional de Cordoba, FONCyT [PICT-2017-1392]; CONICET [PIP-2014, PUE-2016]

    Keywords: Pulegone, Menthone, monolayer; Langmuir film; Molecular dynamics; Mentha components, membrane elasticity

    The insecticidal activity of Mentha oil and its main components has been tested and established for various insects/pests. Several mint ketones have demonstrated to act on GABA(A) receptors (GABA(A)-R), a transmembrane channel target of several important insecticides whose activity can be modulated by surface-active compounds and by changes in the physical properties of the lipid membrane. In the present work, we analyze the capacity of monoterpenic ketones most commonly found in Mentha species, pulegone and menthone, to interact with DPPC membranes by molecular dynamics (MD) simulations and Langmuir monolayers. The experimental results indicate that the presence of menthone and pulegone in the subphase modify the interfacial characteristics of DPPC isotherms. The changes were reflected as expansion of the isotherms and disappearance or bringing forward of DPPC phase transition. MD simulation corroborate these results and indicate that both ketones are located at the region of the carbonyl group, at the interface with the acyl chains. Ketone intercalation between lipid molecules would induce an increasing intermolecular interaction, diminishing the film elasticity and causing an ordering effect. Our results suggest that the insecticidal activity of both ketones could involve their interaction with lipid molecules causing disturbance of the cell membrane as postulated for several larvicide compounds, or at least modulating the receptor surrounding.
    View publication