Contact
    Start Publications Mouthwash containing a biosurfactant and chitosan: An ...
    ←  Publication Finder
    Attension

    Mouthwash containing a biosurfactant and chitosan: An eco-sustainable option for the control of cariogenic microorganisms

    Year: 2019

    Journal: Int. J. Biol. Macromol., Volume 129, MAY 15, page 853–860

    Authors: Farias, Juliana M.; Stamford, Thayza Christina M.; Resende, Ana Helena M.; Aguiar, Jaciana S.; Rufino, Raquel D.; Luna, Juliana M.; Sarubbo, Leonie A.

    Organizations: State of Pernambuco Assistance to Science and Technology Foundation (FACEPE); National Council for Scientific and Technological Development (CNN)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ); Coordination for the Advancement of Higher Education Personnel (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES); National Electrical Energy Agency (ANEEL)

    Keywords: Biosurfactant; Chitosan; Mentha piperita essential oil; Cosmetic; Antimicrobial activity

    The aim of the present study was to determine the antimicrobial action and toxicity of mouthwashes formulated with a biosurfactant, chitosan of a microbial origin and peppermint (Mentha piperita) essential oil (POE). Chitosan was extracted from the biomass of a fungus from the order Mucorales grown in yam bean broth. Three biosurfactants produced by Pseudomonas aeruginosa UCP 0992 (PB), Bacillus cereus UCP 1615 (BB) and Candida bombicola URM 3718 (CB) were tested. Six mouthwashes were prepared, the active ingredients of which were the biosurfactant, chitosan and POE. The minimum inhibitory concentration (MIC) was determined for the test substances separately, in combinations and in the mouthwash formulas. The toxicity of the mouthwashes was tested using MIT (3-(4,5-dimethylthiazole-2-il)-2,5-diphenyltetrazolium bromide) for the L929 (mouse fibroblast) and RAW 264.7 (mouse macrophage) cell lines. All substances tested had a MIC for cariogenic microorganisms. The combinations of the CB and PB biosurfactants with chitosan demonstrated an additive effect on the majority of microorganisms tested. The toxicity of the mouthwashes was significantly lower than that of the commercial mouthwash. The present findings demonstrate that mouthwashes containing natural products constitute a safe, effective, natural alternative to commercially available mouthwashes for the control of oral microorganisms. (C) 2019 Published by Elsevier B.V.
    View publication