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    Multistep building of a soft plant protein film at the air-water interface

    Year: 2018

    Journal: J. Colloid Interface Sci., Volume 526, SEP 15, page 337–346

    Authors: Poirier, Alexandre; Banc, Amelie; Stocco, Antonio; In, Martin; Ramos, Laurence

    Organizations: Laboratory of Excellence NUMEV [ANR-10-LAB-20]

    Keywords: Gliadin; Soft plant protein; Air-water interface; Tensiometry; Dilatational rheology; Ellipsometry; Master curve; Conformational change

    Gliadins are edible wheat storage proteins well known for their surface active properties. In this paper, we present experimental results on the interfacial properties of acidic solutions of gliadin studied over 5 decades of concentrations, from 0.001 to 110 g/L. Dynamic pendant drop tensiometry reveals that the surface pressure Pi of gliadin solutions builds up in a multistep process. The series of curves of the time evolution of Pi collected at different bulk protein concentrations C can be merged onto a single master curve when Pi is plotted as a function of alpha t where t is the time elapsed since the formation of the air/water interface and alpha is a shift parameter that varies with C as a power law with an exponent 2. The existence of such time-concentration superposition, which we evidence for the first time, indicates that the same mechanisms govern the surface tension evolution at all concentrations and are accelerated by an increase of the bulk concentration. The scaling of alpha with C is consistent with a kinetic of adsorption controlled by the diffusion of the proteins in the bulk. Moreover, we show that the proteins adsorption at the air/water interface is kinetically irreversible. Correlated evolutions of the optical and elastic properties of the interfaces, as probed by ellipsometry and surface dilatational rheology respectively, provide a consistent physical picture of the building up of the protein interfacial layer. A progressive coverage of the interface by the proteins occurs at low Pi. This stage is followed, at higher Pi, by conformational rearrangements of the protein film, which are identified by a strong increase of the dissipative viscoelastic properties of the film concomitantly with a peculiar evolution of its optical profile that we have rationalized. In the last stage, at even higher surface pressure, the adsorption is arrested; the optical profile is not modified while the elasticity of the interfacial layer dramatically increases with the surface pressure, presumably due to the film ageing. (C) 2018 Elsevier Inc. All rights reserved.
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