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    Nanotopography reveals metabolites that maintain the immunomodulatory phenotype of mesenchymal stromal cells

    Year: 2023

    Journal: Nature Communications, Volume 14, 2023-02-10, page 753

    Authors: Ross, Ewan A.; Turner, Lesley-Anne; Donnelly, Hannah; Saeed, Anwer; Tsimbouri, Monica P.; Burgess, Karl V.; Blackburn, Gavin; Jayawarna, Vineetha; Xiao, Yinbo; Oliva, Mariana A. G.; Willis, Jennifer; Bansal, Jaspreet; Reynolds, Paul; Wells, Julia A.; Mountford, Joanne; Vassalli, Massimo; Gadegaard, Nikolaj; Oreffo, Richard O. C.; Salmeron-Sanchez, Manuel; Dalby, Matthew J.

    Keywords: Biomaterials; Biomedical materials; Cell signalling; Mesenchymal stem cells; Nanoscale materials

    Mesenchymal stromal cells (MSCs) are multipotent progenitor cells that are of considerable clinical potential in transplantation and anti-inflammatory therapies due to their capacity for tissue repair and immunomodulation. However, MSCs rapidly differentiate once in culture, making their large-scale expansion for use in immunomodulatory therapies challenging. Although the differentiation mechanisms of MSCs have been extensively investigated using materials, little is known about how materials can influence paracrine activities of MSCs. Here, we show that nanotopography can control the immunomodulatory capacity of MSCs through decreased intracellular tension and increasing oxidative glycolysis. We use nanotopography to identify bioactive metabolites that modulate intracellular tension, growth and immunomodulatory phenotype of MSCs in standard culture and during larger scale cell manufacture. Our findings demonstrate an effective route to support large-scale expansion of functional MSCs for therapeutic purposes.
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