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    Non-covalent small molecule partnership for redox-active films: Beyond polydopamine technology

    Journal: J. Colloid Interface Sci., Volume 624, OCT 15, page 400–410

    Authors: Argenziano, Rita; Alfieri, Maria Laura; Arntz, Youri; Castaldo, Rachele; Liberti, Davide; Monti, Daria Maria; Gentile, Gennaro; Panzella, Lucia; Crescenzi, Orlando; Ball, Vincent; Napolitano, Alessandra; d'Ischia, Marco

    Organizations: 2017YJMPZN PRIN

    Keywords: Redox active films; UVA absorbing films; Yellowish homogeneous films; pH dependent hydrophobicity; Non-covalent interactions; Methyl 5; 6-dihydroxyindole-2-carboxylate; Hexamethylenediamine; Film component structural analysis; Reducing activity; UV stressed HaCaT cells

    Hypothesis: The possibility to use hexamethylenediamine (HMDA) to impart film forming ability to natural polymers including eumelanins and plant polyphenols endowed with biological activity and functional properties has been recently explored with the aim to broaden the potential of polydopamine (PDA)-based films overcoming their inherent limitations. 5,6-dihydroxyindole-2-carboxylic acid, its methyl ester (MeDHICA) and eumelanins thereof were shown to exhibit potent reducing activity. Experiments: MeDHICA and HMDA were reacted in aqueous buffer, pH 9.0 in the presence of different substrates to assess the film forming ability. The effect of different reaction parameters (pH, diamine chain length) on film formation was investigated. Voltammetric and AFM /SEM methods were applied for analysis of the film redox activity and morphology. HPLC, MALDI-MS and 1HNMR were used for chemical characterization. The film reducing activity was evaluated in comparison with PDA by chemical assays and using UV stressed human immortalized keratinocytes (HaCat) cells model. Findings: Regular and homogeneous yellowish films were obtained with moderately hydrophobic properties. Film deposition was optimal at pH 9, and specifically induced by HMDA. The film consisted of HMDA and monomeric MeDHICA accompanied by dimers/small oligomers, but no detectable
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