Nonionic surfactant-based cloud point extraction of polyhydroxyalkanoate from the fermentation crude in a rotating disc contactor
Year: 2021
Journal: Sep. Sci. Technol., Volume 56, JUN 13, page 1534–1545
Authors: Murugesan, Sivananth; Iyyaswami, Regupathi; J. Khandelwal, Palash
Keywords: Cloud point extraction; polyhydroxyalkanoate; rotating disc contactor; Cupriavidus necator
Nonionic surfactant-based cloud point extraction of polyhydroxyalkanoate from fermentation broth was implemented in a modified rotating disc contactor. The effect of rotor speed and phase flowrates on dispersed micellar phase holdup, mass transfer coefficient, yield, and purity were studied with micellar system contains TX114 (4.5 wt %)+ TMN6 (0.5 wt %) and 0.1 M ammonium chloride at broth pH of 3. The rotor speed and the micellar phase flowrate had a significant effect. A maximum PHA recovery of 85.48% and purity of 86.01% were achieved. The correlation was developed to predict the dispersed-phase holdup.
