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    Novel and rapid activation of polyvinylidene fluoride membranes by UV light

    Year: 2019

    Journal: React. Funct. Polym., Volume 140, JUL, page 56–61

    Authors: Jimenez-Meneses, Pilar; Banuls, Maria-Jose; Puchades, Rosa; Maquieira, Angel

    Organizations: Spanish Ministry of Economy and Competitiveness (BIHOLOG) [CTQ2016-75749-R]; FEDEREuropean Commission; Spanish Ministry of Economy, Industry and Competitiveness [CTQ2013-45875-R]; European Social FundEuropean Social Fund (ESF)

    Keywords: PVDF membranes; Hydroxylation; Silanization; Photoinduced immobilization; Nucleic acid microarrays

    Polyvinylidene fluoride (PVDF) membranes have become essential because of their huge applicability to the industry; however, they still present some limitations. This study focuses on the modification of PVDF membrane properties such as hydrophobicity, wettability, and functionality. To obtain a stable grafting, the surface of the membrane is hydroxylated using UV light at 254 nm, followed by covalent immobilization of (3-aminopropyl) triethoxysilane (APTES) and vinyltriethoxysilane (VMS). The physicochemical and morphological properties of modified and raw PVDF membranes were analyzed by spectroscopy, microscopy, and goniometry. Finally, nucleic acid microarray technology results showed that PVDF and PVDF-VTES membranes had probe immobilization densities of 5 and 11 pmol/cm(2) and hybridization limits of detection of 1 and 5 nM, respectively.
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