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    Novel production of biodispersant by Serratia marcescens UCP 1549 in solid-state fermentation and application for oil spill bioremediation

    Year: 2022

    Journal: Environ. Technol., Volume 43, AUG 24, page 2956–2967

    Authors: dos Santos, Renata Andreia; Rodriguez, Dayana Montero; da Silva Ferreira, Isabela Natalia; de Almeida, Sergio Mendonca; de Campos Takaki, Galba Maria; Barbosa de Lima, Marcos Antonio

    Organizations: CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior) [88887.342878/2019-00]; CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [314422/2018-8]; FACEPE (Fundacao de Amparo a Ciencia e Tecnologia de Pernambuco) [0291-2.12/15]

    Keywords: Oil spill bioremediation; biosurfactant; static fermentation; agro-industrial substrate; oil displacement area

    Oil spills in aquatic ecosystems cause irreparable damage to marine life and the coastal populations of affected areas. In recent years, chemical dispersants have been extensively used to remedy these impacted ecosystems, although these agents have been increasingly restricted due to their toxic potential. In this context, biosurfactants are emerging as a promising alternative to chemical dispersants, which have some advantages including low toxicity, high biodegradability and good ecological acceptability. Thus, this study aimed to the production of biosurfactant by the bacteria Serratia marcescens UCP 1549 for application as biodispersant. The experiment was carried out using wheat bran as substrate in solid-state fermentation (SSF) as low-cost technology. Biosurfactant production was verified by the reduction of surface tension (28.4 mN/m) and interfacial tension (4.1 mN/m) with n-hexadecane. Also, promising result of emulsification (94%) with burned motor oil was obtained. Acid precipitation yielded 52.0 g/kg dry substrate of biosurfactant, that was identified as an anionic compound of a lipopeptide nature by the Zeta potential and FTIR spectrum, respectively. The biomolecule showed stability under extreme conditions of temperature, pH and salinity, as well as low toxicity against the microcrustacean Artemia salina. In addition, the biosurfactant demonstrated excellent properties to dispersing burned motor oil in water (ODA = 50.24 cm(2)) and to washing of marine stones (100% removal of burned motor oil). Therefore, these results confirm SSF as a sustainable technology for the production of biodispersant by S. marcescens UCP 1549, promising in the bioremediation of marine ecosystems impacted by petroderivatives.
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