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    Preparation and Characterization of Porous Scaffolds Based on Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

    Year: 2021

    Journal: Life-Basel, Volume 11, SEP

    Authors: Esmail, Asiyah; Pereira, Joao R.; Sevrin, Chantal; Grandfils, Christian; Menda, Ugur Deneb; Fortunato, Elvira; Oliva, Abel; Freitas, Filomena

    Organizations: FCT-Fundacao para a Ciencia e a Tecnologia, I.P. of the Research Unit on Applied Molecular Biosciences-UCIBIO [UIDP/04378/2020, UIDB/04378/2020]; Associate Laboratory Institute for Health and Bioeconomy-i4HB [LA/P/0140/2020]; Instituto de Tecnologia Quimica e Biologica Antonio Xavier (ITQB NOVA) [iNOVA4Health/MULTI/04462/2018]; Materials Research Center from the Associate Laboratory [UIDB/50025/2020]; FCT I.P. [SFRH/BD/147518/2019]

    Keywords: Poly(3-hydroxybutyrate); P(3HB); Poly(3-hydroxybutyrate-co-3-hydroxyvalerate; P(3HB-co-3HV); porous scaffold; salt leaching; emulsion templating

    Poly(hydroxyalkanoates) (PHAs) with different material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), and the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate, P(3HB-co-3HV), with a 3HV of 25 wt.%, were used for the preparation of porous biopolymeric scaffolds. Solvent casting with particulate leaching (SCPL) and emulsion templating were evaluated to process these biopolymers in porous scaffolds. SCPL scaffolds were highly hydrophilic (>170% swelling in water) but fragile, probably due to the increase of the polymer's polydispersity index and its high porosity (>50%). In contrast, the emulsion templating technique resulted in scaffolds with a good compromise between porosity (27-49% porosity) and hydrophilicity (>30% water swelling) and without impairing their mechanical properties (3.18-3.35 MPa tensile strength and 0.07-0.11 MPa Young's Modulus). These specifications are in the same range compared to other polymer-based scaffolds developed for tissue engineering. P(3HB-co-3HV) displayed the best overall properties, namely, lower crystallinity (11.3%) and higher flexibility (14.8% elongation at break. Our findings highlight the potency of our natural biopolyesters for the future development of novel porous scaffolds in tissue engineering, thanks also to their safety and biodegradability.
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