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    Pristine and Antibiotic-Loaded Nanosheets/Nanoneedles-Based Boron Nitride Films as a Promising Platform to Suppress Bacterial and Fungal Infections

    Year: 2020

    Journal: ACS Appl. Mater. Interfaces, Volume 12, SEP 23, page 42485–42498

    Authors: Gudz, Kristina Y.; Permyakova, Elizaveta S.; Matveev, Andrei T.; Bondarev, Andrey, V; Manakhov, Anton M.; Sidorenko, Daria A.; Filippovich, Svetlana Y.; Brouchkov, Anatoli, V; Golberg, Dmitri, V; Ignatov, Sergei G.; Shtansky, Dmitry, V

    Organizations: Russian Science FoundationRussian Science Foundation (RSF) [20-19-00120]; Ministry of Education and Science of the Russian Federation (Increase Competitiveness Program of NUST "MISiS") [K2A-2018-038]; grant "Novel nanostructures for engineering applications" [CZ.02.1.01/0.0/0.0/16_026/0008396]; MEYS CR [LM2018110]; Australian Research Council (ARC)Australian Research Council [FL160100089]

    Keywords: nanostructured BN-based films; antibiotic-loaded surfaces; drug release; antibacterial activity; antifungal activity

    In recent years, bacteria inactivation during their direct physical contact with surface nanotopography has become one of the promising strategies for fighting infection. Contact-killing ability has been reported for several nanostructured surfaces, e.g., black silicon, carbon nanotubes, zinc oxide nanorods, and copper oxide nanosheets. Herein, we demonstrate that Gram-negative antibiotic-resistant Escherichia coli (E. coli) bacteria are killed as a result of their physical destruction while contacting nanostructured h-BN surfaces. BN films, made of spherical nanoparticles formed by numerous nanosheets and nanoneedles with a thickness <15 nm, have been obtained through a reaction of ammonia with amorphous boron. The contact-killing bactericidal effect of BN nanostructures has been compared with a toxic effect of gentamicin released from them. For a wider protection against bacterial and fungal infection, the films have been saturated with a mixture of gentamicin and amphotericin B. Such BN films demonstrate a high antibiotic/antimycotic agent loading capacity and a fast initial and sustained release of therapeutic agents for 170-260 h depending on the loaded dose. The pristine BN films possess high antibacterial activity against E. coli K-261 strain at their initial concentration of 10(4) cells/mL, attaining >99% inactivation of colony forming units after 24 h, same as gentamicin-loaded (150 mu g/cm(2)) BN sample. The BN films loaded with a mixture of gentamicin (150 and 300 mu g/cm(2)) and amphotericin B (100 mu g/cm(2)) effectively inhibit the growth of E. coli K-261 and Neurospora crassa strains. During immersion in the normal saline solution, the BN film generates reactive oxygen species (ROS), which can lead to accelerated oxidative stress at the site of physical cell damage. The obtained results are valuable for further development of nanostructured surfaces having contact killing, ROS, and biocide release abilities.
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