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    Production, formulation and cost estimation of a commercial biosurfactant

    Year: 2019

    Journal: Biodegradation, Volume 30, AUG, page 191–201

    Authors: Freire Soares da Silva, Rita de Cassia; de Almeida, Darne Germano; Ferreira Brasileiro, Pedro Pinto; Rufino, Raquel Diniz; de Luna, Juliana Moura; Sarubbo, Leonie Asfora

    Organizations: National Agency of Electrical Energy (ANEEL); Thermoelectric EPASA (Centrais Eletricas da Paraiba); Foundation for the Support of Science and Technology of the State of Pernambuco (FACEPE)Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE); National Council for Scientific and Technological Development (CNPq)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ); Coordination for the Improvement of Higher Level Education Personnel (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)

    Keywords: Biosurfactants price; Pseudomonas cepacia CCT6659; Formulation; Scale up; Cost estimation

    Due to their amphipathic nature, biosurfactants are multifunctional molecules that have considerable potential in several industries, especially the petroleum industry. In this study, the commercial production of a biosurfactant from Pseudomonas cepacia CCT6659 grown on industrial waste was investigated in a semi-industrial 50-L bioreactor for use in the removal of hydrocarbons from oily effluents. A concentration of 40.5g/L was achieved in the scale up and the surface tension was reduced to 29 mN/m. The biosurfactant was formulated with an added preservative, tyndallization and the combination of fluent vaporization plus the preservative. Formulated biosurfactant samples were stored for 120days. Tensioactive properties and stability were evaluated with different pH values, temperatures and salt concentrations. The commercial biosurfactant obtained with all formulation methods demonstrated good stability, with tolerance to a wide range of pH values as well as high temperature and high salinity, enabling application in extreme environmental conditions, as it occurs in industrial plants. The biosurfactant proved to be economically viable for large-scale application, as demonstrated by the cost of the product, estimated at around US$ 0.14-0.15/L and US$ 0.02/g for the formulated and the isolated biosurfactant, respectively. Both products were applied in an oil-fired thermoelectric plant for the treatment of oily effluents and removed up to 100% of the oil. Therefore, this biosurfactant is suitable for application under extreme conditions, such as in the petroleum industry, and can be produced at a more attractive price compared to other commercially available products on the market.
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