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    Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading AntarcticRhodococcus

    Year: 2020

    Journal: Int. J. Mol. Sci., Volume 21, SEP

    Authors: Habib, Syahir; Ahmad, Siti Aqlima; Wan Johari, Wan Lutfi; Abd Shukor, Mohd Yunus; Alias, Siti Aisyah; Smykla, Jerzy; Saruni, Nurul Hani; Abdul Razak, Nur Syafiqah; Yasid, Nur Adeela

    Organizations: Putra Young Initiative (IPM) under Universiti Putra Malaysia [9476900]; Putra Graduate Initiative (IPS) under Universiti Putra Malaysia [9508500]

    Keywords: surface-active lipopeptide; biosynthetic gene clusters; fellfield soil; Antarctica

    Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted onRhodococcussp. ADL36, a diesel-degrading Antarctic bacterium. The strain showed a positive bacterial adhesion to hydrocarbon (BATH) assay, drop collapse test, oil displacement activity, microplate assay, maximal emulsification index at 45% and ability to reduce water surface tension to < 30 mN/m. The evaluation of the cell-free supernatant demonstrated its high stability across the temperature, pH and salinity gradient although no correlation was found between the surface and emulsification activity. Based on the positive relationship between the assessment of macromolecules content and infrared analysis, the extracted biosurfactant synthesized was classified as a lipopeptide. Prediction of the secondary metabolites in the non-ribosomal peptide synthetase (NRPS) clusters suggested the likelihood of the surface-active lipopeptide production in the strain's genomic data. This is the third report of surface-active lipopeptide producers from this phylotype and the first from the polar region. The lipopeptide synthesized by ADL36 has the prospect to be an Antarctic remediation tool while furnishing a distinctive natural product for biotechnological application and research.
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