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    Profiling Heparan Sulfate-Heavy Metal Ions Interaction Using Electrochemical Techniques

    Year: 2022

    Journal: Chem.-Eur. J., Volume 28, OCT 4

    Authors: Shitrit, Ariel; Mardhekar, Sandhya; Alshanski, Israel; Jain, Prashant; Raigawali, Rakesh; Shanthamurthy, Chethan D.; Kikkeri, Raghavendra; Yitzchaik, Shlomo; Hurevich, Mattan

    Organizations: Hebrew University Center for Nanoscience and Nanotechnology Ph.D. Scholarship; COST Action [18103 INNOGLY]; European Innovation Council (EIC) under the European Union's Horizon Europe research and innovation program [101046369]; IISER, Pune [STARS/APR2019/CS/426/FS, SERB/F/9228/2019-2020]

    Keywords: carbohydrates; electrochemistry; heavy metal ions; sulfation pattern; surface chemistry; uronic acid

    Heparan sulfate glycosaminoglycans provides extracellular matrix defense against heavy metals cytotoxicity. Identifying the precise glycan sequences that bind a particular heavy metal ion is a key for understanding those interactions. Here, electrochemical and surface characterization techniques were used to elucidate the relation between the glycans structural motifs, uronic acid stereochemistry, and sulfation regiochemistry to heavy metal ions binding. A divergent strategy was employed to access a small library of structurally well-defined tetrasaccharides analogs with different sulfation patterns and uronic acid compositions. These tetrasaccharides were electrochemically grafted onto glassy carbon electrodes and their response to heavy metal ions was monitored by electrochemical impedance spectroscopy. Key differences in the binding of Hg(II), Cd(II), and Pb(II) were associated with a combination of the uronic acid type and the sulfation pattern.
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