Contact
    Start Publications Programmable multi-DNA release from multilayered ...
    ←  Publication Finder
    QSense

    Programmable multi-DNA release from multilayered polyelectrolytes using gigahertz nano-electromechanical resonator

    Year: 2019

    Journal: J. Nanobiotechnol., Volume 17, AUG 6

    Authors: Guo, Xinyi; Zhang, Hongxiang; Wang, Yanyan; Pang, Wei; Duan, Xuexin

    Keywords: Controllable release; NEMS resonator; Gigahertz ultrasound; Acoustic streaming; Micro-vortexes; Polyelectrolyte thin films

    Background Controllable and multiple DNA release is critical in modern gene-based therapies. Current approaches require complex assistant molecules for combined release. To overcome the restrictions on the materials and environment, a novel and versatile DNA release method using a nano-electromechanical (NEMS) hypersonic resonator of gigahertz (GHz) frequency is developed. Results The micro-vortexes excited by ultra-high frequency acoustic wave can generate tunable shear stress at solid-liquid interface, thereby disrupting molecular interactions in immobilized multilayered polyelectrolyte thin films and releasing embedded DNA strands in a controlled fashion. Both finite element model analysis and experiment results verify the feasibility of this method. The release rate and released amount are confirmed to be well tuned. Owing to the different forces generated at different depth of the films, release of two types of DNA molecules with different velocities is achieved, which further explores its application in combined gene therapy. Conclusions Our research confirmed that this novel platform based on a nano-electromechanical hypersonic resonator works well for controllable single and multi-DNA release. In addition, the unique features of this resonator such as miniaturization and batch manufacturing open its possibility to be developed into a high-throughput, implantable and site targeting DNA release and delivery system.
    View publication