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    Rapid Point-of-Care Electrochemical Sensor for the Detection of Cancer Tn Antigen Carbohydrate in Whole Unprocessed Blood

    Year: 2022

    Journal: ACS Sens., Volume 7, NOV 25, page 3379–3388

    Authors: Silva, Saimon M.; Langley, Daniel P.; Cossins, Luke R.; Samudra, Anushka N.; Quigley, Anita F.; Kapsa, Robert M. I.; Tothill, Richard W.; Greene, George W.; Moulton, Simon E.

    Organizations: Australian Research Council; Lubris Biopharma; [DP180102287]

    Keywords: Tn antigen; lubricin; cancer biomarkers; lectin-based diagnostics; point-of-care electrochemical sensor; antifouling coatings; abnormal O-glycans; liquid biopsies

    Improving outcomes for cancer patients during treatment and monitoring for cancer recurrence requires personal-ized care which can only be achieved through regular surveillance for biomarkers. Unfortunately, routine detection for blood-based biomarkers is cost-prohibitive using currently specialized laborato-ries. Using a rapid self-assembly sensing interface amenable to methods of mass production, we demonstrate the ability to detect and quantify a small carbohydrate-based cancer biomarker, Tn antigen (alpha GalNAc-Ser/Thr) in a small volume of blood, using a test format strip reminiscent of a blood glucose test. The detection of Tn antigen at picomolar levels is achieved through a new transduction mechanism based on the impact of Tn antigen interactions on the molecular dynamic motion of a lectin cross-linked lubricin antifouling brush. In tests performed on retrospective blood plasma samples from patients presenting three different tumor types, differentiation between healthy and diseased patients was achieved, highlighting the clinical potential for cancer monitoring.
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