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    Resveratrol-Loaded Hydrogel Contact Lenses with Antioxidant and Antibiofilm Performance

    Year: 2021

    Journal: Pharmaceutics, Volume 13, APR

    Authors: Vivero-Lopez, Maria; Muras, Andrea; Silva, Diana; Serro, Ana Paula; Otero, Ana; Concheiro, Angel; Alvarez-Lorenzo, Carmen

    Organizations: MINECO [SAF2017-83118-R]; Agencia Estatal de Investigacion (AEI) Spain; Xunta de Galicia [ED431C 2020/17]; FEDER; Fundacao para a Ciencia e Tecnologia (FCT) Portugal [UIDB/00100/2020, UIDB/04585/2020]; Xunta de Galicia (Conselleria de Cultura, Educacion e Ordenacion Universitaria) [ED481A-2019/120]

    Keywords: antibiofilm; antioxidant; drug-eluting contact lenses; microbial keratitis; endophthalmitis; device-related ocular infections

    Contact lenses (CLs) are prone to biofilm formation, which may cause severe ocular infections. Since the use of antibiotics is associated with resistance concerns, here, two alternative strategies were evaluated to endow CLs with antibiofilm features: copolymerization with the antifouling monomer 2-methacryloyloxyethyl phosphorylcholine (MPC) and loading of the antioxidant resveratrol with known antibacterial activity. MPC has, so far, been used to increase water retention on the CL surface (Proclear(R) 1 day CLs). Both poly(hydroxyethyl methacrylate) (HEMA) and silicone hydrogels were prepared with MPC covering a wide range of concentrations (from 0 to 101 mM). All hydrogels showed physical properties adequate for CLs and successfully passed the hen's egg-chorioallantoic membrane (HET-CAM) test. Silicone hydrogels had stronger affinity for resveratrol, with higher loading and a slower release rate. Ex vivo cornea and sclera permeability tests revealed that resveratrol released from the hydrogels readily accumulated in both tissues but did not cross through. The antibiofilm tests against Pseudomonas aeruginosa and Staphylococcus aureus evidenced that, in general, resveratrol decreased biofilm formation, which correlated with its concentration-dependent antibacterial capability. Preferential adsorption of lysozyme, compared to albumin, might also contribute to the antimicrobial activity. In addition, importantly, the loading of resveratrol in the hydrogels preserved the antioxidant activity, even against photodegradation. Overall, the designed hydrogels can host therapeutically relevant amounts of resveratrol to be sustainedly released on the eye, providing antibiofilm and antioxidant performance.
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