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    RGD-functionalization of PLA/starch scaffolds obtained by electrospinning and evaluated in vitro for potential bone regeneration

    Year: 2019

    Journal: Mater. Sci. Eng. C-Mater. Biol. Appl., Volume 96, MAR, page 798–806

    Authors: Gutierrez-Sanchez, Mariana; Escobar-Barrios, Vladimir A.; Pozos-Guillen, Amaury; Escobar-Garcia, Diana M.

    Organizations: CONACYTConsejo Nacional de Ciencia y Tecnologia (CONACyT) [291159]

    Keywords: Poly (lactic acid); Starch; RGD; Scaffolds; Electrospinning; In vitro evaluation

    The engineering of bone tissues represents an area of opportunity for the development of new polymeric compounds. In this context, the objective of this work is the generation and evaluation in vitro of supports obtained from mixtures of starch with poly (lactic acid) (PLA), treated with arginine-glycine-aspartic acid peptides (RGD). For this, non-woven fibers of PLA with different starch content (0.0, 2.5, 5.0 and 10.0%wt) were obtained using the electrospinning technique. Then the physical absorption of RGD was carried out, with the aim of increasing the cellular adhesion of the polymeric material. Subsequently, in vitro biocompatibility tests were performed, and viability (LIFE/DEAD), proliferation (MTS assay) and cell adhesion were carried out with osteoblasts incubated for 48 h. Regarding biocompatibility results, only viable cells were found for all the compositions, and the biocompatibility of the materials was validated by the morphological analysis of the cultured cells, where extended cells were observed. Proliferation assays show that osteoblasts proliferate better on the surfaces of PLA and PLA with 5.0% starch scaffolds. Therefore, it is concluded that the scaffolds obtained by electrospinning of PLA with starch and functionalized with RGD are promising for its use in the regeneration of bone tissue.
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