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    ss-CD-Functionalized Microdevice for Rapid Capture and Release of Bacteria

    Year: 2017

    Journal: ACS Appl. Mater. Interfaces, Volume 9, APR 26, page 13928–13938

    Authors: Perez-Anes, Alexandra; Szarpak-Jankowska, Anna; Jary, Dorothee; Auzely-Velty, Rachel

    Keywords: cyclodextrin; bacterial capture; quartz crystal microbalance; microfluidic chip; host-guest interaction

    Most procedures for detecting pathogens in liquid media require an initial concentration step. In this regard, carbohydrates have proven to be attractive affinity ligands for the solid-phase capture of bacteria that use lectins for adhesion to host cell membranes. However, the use of cyclodextrin-immobilized substrates to selectively trap bacteria has not been explored before. Here, using, quartz-crystal microbalance with dissipation monitoring experiments, we demonstrate that functionalization of surfaces by ss-cydodextrin (ss-CD) can not only allow for rapid and efficient capture of bacterial cells in liquid but also their facile elution with an aqueous solution of a selectively methylated ss-CD derivative as a competitive molecule. This capture/ elution strategy, which is based on host guest interactions between membrane components of the bacterial cell and the. CD cavities, is performed in physiological conditions and can be integrated in a microchip. Indeed, proof-of-concept studies showed the potential of ss-CD-modified micropillar-integrated microfluidic devices for concentration of bacteria. The results obtained with Escherichia coli suggest that this approach could be broadly applicable among Gram-negative bacteria, which share common cell membrane structures.
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