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    Star polymer-based nanolayers with immobilized complexes of polycationic stars and DNA for deposition gene delivery and recovery of intact transfected cells

    Year: 2020

    Journal: Int. J. Pharm., Volume 589, NOV 15

    Authors: Mendrek, Barbara; Fus-Kujawa, Agnieszka; Teper, Paulina; Botor, Malwina; Kubacki, Jerzy; Sieron, Aleksander L.; Kowalczuk, Agnieszka

    Organizations: Polish National Science Center [UM0-2015/17/B/ST5/01095]

    Keywords: Star polymer nanolayers; Thermoresponsive polymers; Polyplexes; Deposition transfection; DNA delivery

    We designed a novel thermoresponsive system of nanolayers composed of star poly [oligo(ethylene glycol) methacrylate]s (S-POEGMA) covalently bonded to a solid support and covered with polyplexes of cationic star polymers and plasmid DNA (pDNA). S-POEGMA stars were attached to the solid support via a UV-mediated "grafting to" method. To the best of our knowledge, for the first time, the conformational changes of obtained star nanolayers, occurring with changes in temperature, were studied using a quartz crystal microbalance technique. Next, the polyplexes of star poly [N,N'-dimethylaminoethyl methacrylate-ran-di(ethylene glycol) methacrylate] (S-P(DMAEMA-DEGMA)) with pDNA, exhibiting a phase transition temperature (Tcp) in culture medium DMEM, were deposited on S-POEGMA layers when the temperature increased above the Tcp of polyplex. The thermoresponsivity of the system was then the main mechanism for controlling the adhesion, proliferation, transfection and detachment of HT-1080 cells. The nanolayers promoted the effective cell culture and delivered nucleic acids into cells, with a transfection efficiency several times higher than that of the control. The detachment of the transfected cells was regulated only by the change of temperature. The studies demonstrated that we obtained a novel and effective system, based on a star polymer architecture, useful for gene delivery and tissue engineering applications.
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