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    Study of the Adhesion of the Human Gut Microbiota on Electrospun Structures

    Year: 2022

    Journal: Bioengineering-Basel, Volume 9, MAR

    Authors: Biagini, Francesco; Calvigioni, Marco; De Maria, Carmelo; Magliaro, Chiara; Montemurro, Francesca; Mazzantini, Diletta; Celandroni, Francesco; Mattioli-Belmonte, Monica; Ghelardi, Emilia; Vozzi, Giovanni

    Organizations: BIOMEMBRANE project (M-ERA.net 2 project) [4246]; KERAPACK project (MANUNET MNET) [17/NMAT-0060]; University of Pisa [PRA_2018_68]

    Keywords: gut microbiota; electrospinning; biofabrication

    Although the adhesion of bacteria on surfaces is a widely studied process, to date, most of the works focus on a single species of microorganisms and are aimed at evaluating the antimicrobial properties of biomaterials. Here, we describe how a complex microbial community, i.e., the human gut microbiota, adheres to a surface to form stable biofilms. Two electrospun structures made of natural, i.e., gelatin, and synthetic, i.e., polycaprolactone, polymers were used to study their ability to both promote the adhesion of the human gut microbiota and support microbial growth in vitro. Due to the different wettabilities of the two surfaces, a mucin coating was also added to the structures to decouple the effect of bulk and surface properties on microbial adhesion. The developed biofilm was quantified and monitored using live/dead imaging and scanning electron microscopy. The results indicated that the electrospun gelatin structure without the mucin coating was the optimal choice for developing a 3D in vitro model of the human gut microbiota.
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