Contact
    Start Publications Succinimidyl Carbonate-Based Amine-Reactive Polymer Brushes: ...
    ←  Publication Finder
    Attension

    Succinimidyl Carbonate-Based Amine-Reactive Polymer Brushes: Facile Fabrication of Functional Interfaces

    Year: 2021

    Journal: ACS Appl. Polym. Mater., Volume 3, MAY 14, page 2507–2517

    Authors: Gevrek, Tugce Nihal; Degirmenci, Aysun; Sanyal, Rana; Klok, Harm-Anton; Sanyal, Amitav

    Organizations: Presidency of Republic of Turkey Directorate of Strategy and Budget [2009K120520]

    Keywords: polymeric coatings; biofunctionalization; polymer brushes; succinimidyl carbonate; carbamate formation; amine conjugation

    Polymeric coatings that can undergo effective functionalization with ligands and (bio)molecules necessary for intended applications are widely employed to engineer functional interfaces. Herein, amine-reactive polymer brushes are fabricated using a succinimidyl group-activated carbonate monomer, and their facile post-polymerization functionalization is demonstrated through ligand-mediated protein immobilization and detection. Copolymer brushes containing varying amounts of the reactive monomer and an ethylene glycol-based methacrylate comonomer were obtained using surface-mediated reversible addition fragmentation chain transfer polymerization. Post-polymerization functionalization of the thus-obtained copolymer brushes with a fluorine-containing amine, namely, 4-(trifluoromethyl)benzylamine, demonstrated highly efficient functionalization at ambient temperature. To demonstrate possible applications, polymer brushes functionalized with a bioactive ligand, namely, biotin, were used to detect the target protein, streptavidin. The biotin-streptavidin interaction on brushes could also be employed to conjugate protein-coated quantum dots. Importantly, comparison of the attachment of 4-(trifluoromethyl)benzylamine on activated carbonate group-containing brushes with reaction of the same molecule on traditional active ester-based brushes demonstrated higher extent of conjugation to carbonate brushes. Finally, orthogonal functionalization of the copolymer brushes with an amino-functionalized molecule and a maleimide-containing fluorescent dye in spatial control using microcontact printing was demonstrated. One can envision that the facile fabrication and efficient functionalization of succinimidyl carbonate-based amine-reactive brushes afford an attractive platform for applications using functional interfaces for diagnostic purposes.
    View publication