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    Surface chemistry and spectroscopic studies of the native phenylalanine dehydrogenase Langmuir monolayer at the air/aqueous NaCl interface

    Year: 2020

    Journal: J. Colloid Interface Sci., Volume 560, FEB 15, page 458–466

    Authors: da Silva, RLCG; Sharma, SK; Paudyal, S; Mintz, KJ; Caseli, L; Leblanc, RM

    Organizations: Sao Paulo Research Foundation (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2019/01797-6]; University of Miami (Miami -Florida); Federal University of Sao Paulo (Diadema -Sao Paulo)

    Keywords: Phenylalanine dehydrogenase; Langmuir monolayer; Surface chemistry; Reflectance-absorbance infrared spectroscopy; Langmuir-Blodgett film

    This study investigates the main aspects of the surface behavior of the native phenylalanine dehydrogenase (PheDH) enzyme at the air/aqueous interface employing a saline subphase to induce the enzyme surface activity. Surface chemistry experiments were performed in order to determine the surface packing and stability of the formed layer, while spectroscopic experiments provided information regarding its secondary structure conformation. It was found that the PheDH enzyme forms a fluid film, which is quite homogeneous throughout its entire compression, being stable for long periods of time with no significant evidence of aggregates or irreversible domains during interfacial compression/decompression processes. The main secondary structures of the interfacial PheDH film were accessed via in situ reflectance-absorbance infrared spectroscopy, indicating a majority presence of oc-helices, which were maintained after the film transfer to solid muscovite supports. The immobilized films presented a homogeneous and regular deposition, with controlled roughness and a mean thickness in the range of 8-10 nm. (C) 2019 Elsevier Inc. All rights reserved.
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