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    Surface-Functionalized Polystyrene Nanoparticles Alter the Transmembrane Potential via Ion-Selective Pores Maintaining Global Bilayer Integrity

    Year: 2022

    Journal: Langmuir

    Authors: Aurora Perini, D.; Parra-Ortiz, Elisa; Varo, Inmaculada; Queralt-Martin, Maria; Malmsten, Martin; Alcaraz, Antonio

    Organizations: Spanish Government MCIN/AEI [2019-108434GB-I00, IJC2018-035283-I]; Universitat Jaume I [UJI-B2018-53, UJI-A2020-21]; Generalitat Valenciana [GRISOLIAP/2018/061, AICO/2020/066, BEFPI/2020/040]; Swedish Research Council [2016-05157, 2021-05498]; Independent Research Fund Denmark [9040-00020B]; LEO Foundation Center for Cutaneous Drug Delivery [2016-11-01]

    Although nanoplastics have well-known toxic effects toward the environment and living organisms, their molecular toxicity mechanisms, including the nature of nanoparticle-cell membrane interactions, are still under investigation. Here, we employ dynamic light scattering, quartz crystal microbalance with dissipation monitoring, and electrophysiology to investigate the interaction between polystyrene nanoparticles (PS NPs) and phospholipid membranes. Our results show that PS NPs adsorb onto lipid bilayers creating soft inhomogeneous films that include disordered defects. PS NPs form an integral part of the generated channels so that the surface functionalization and charge of the NP determine the pore conductive properties. The large difference in size between the NP diameter and the lipid bilayer thickness (similar to 60 vs similar to 5 nm) suggests a particular and complex lipid-NP assembly that is able to maintain overall membrane integrity. In view of this, we suggest that NP-induced toxicity in cells could operate in more subtle ways than membrane disintegration, such as inducing lipid reorganization and transmembrane ionic fluxes that disrupt the membrane potential.
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