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    Surface interactions determined by stereostructure on the example of 7-hydroxycholesterol epimers - The Langmuir monolayer study

    Year: 2019

    Journal: Biochim. Biophys. Acta-Biomembr., Volume 1861, 1-Jul, page 1275–1283

    Authors: Chachaj-Brekiesz, Anna; Wnetrzak, Anita; Lipiec, Ewelina; Dynarowicz-Latka, Patrycja

    Keywords: Langmuir monolayers; Oxysterols; Stereoselectivity; Epimers; Interactions

    Stereoselective interactions are pivotal for molecular recognition between biomolecules and lipid surfaces. The aim of this study was to determine factors differencing molecular interactions between 7-hydroxycholesterol epimers (oxysterols, which excessively appear in pathological processes in human body) and natural membrane phospholipids. Two-component systems of different mutual proportions of 7-hydroxycholesterol (7 alpha-hydroxycholesterol or 7-beta-hydroxycholesterol, in short 7 alpha-OH or 7 beta-OH) and membrane lipids (POPC, DPPC, DPPE, DPPS, SM) were systematically analyzed in artificial membranes modeled as Langmuir monolayers. Classical surface pressure measurements were complemented with direct visualization of films texture both in situ (with Brewster angle microscopy, BAM) and after their transfer onto solid supports (with Atomic Force Microscopy, AFM). Our results clearly show striking differences in surface properties of the studied binary mixtures, emphasizing distinct effects of both 7-hydroxycholesterol epimers on the organization of lipid layers. Systematic study allowed to conclude that the structure of polar head group and interfacial region of the molecule play important role in oxysterol-phospholipid interactions, while the hydrophobic region is significantly less important in this respect.
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