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    Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology

    Year: 2020

    Journal: Biosensors-Basel, Volume 10, MAR

    Authors: Spagnolo, Sandro; De La Franier, Brian; Hianik, Tibor; Thompson, Michael

    Organizations: European Commission within the project Innovative Technology for the Detection of Enzyme Activity in Milk (formilk) [690898/H2020-MSCA-RISE-2015]; Natural Sciences and Engineering Research Council of CanadaNatural Sciences and Engineering Research Council of Canada (NSERC)CGIAR

    Keywords: anti-fouling monolayer; electromagnetic piezoelectric acoustic sensor; blood serum; milk

    This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS) configuration. The MEG-Cl coated surface was both employed with Ni-NTA for the binding of recombinant proteins and for the tandem property of the avoidance of fouling from serum and milk. The MEG-Cl coated surfaces were found to provide a large degree of anti-fouling on the EMPAS device, and were comparable to previously studied MEG-OH surfaces. Importantly, the monolayer continued to provide anti-fouling capability to the biosensor following extension with Ni-NTA in place. Accordingly, this surface linker provides an attractive system for use in biosensor technology in terms of both its anti-fouling and linking properties.
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