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    Synergistic Effects of Lauric Arginate and Peracetic Acid in Reducing Listeria monocytogenes on Fresh Apples

    Year: 2021

    Journal: Front. Microbiol., Volume 12, JUN 18

    Authors: Shen, Xiaoye; Cong, Jian; Mugendi, Joshua; Hanrahan, Ines; Zhu, Mei-Jun

    Organizations: Washington State Department of Agriculture (WSDA); Center for Produce Safety [2017CPS10]; Washington Tree Fruit Research Commission

    Keywords: Listeria monocytogenes; peracetic acid; lauric arginate; apples; spray application; temperature

    Apples are naturally coated with a water-repelling hydrophobic wax layer, which may limit the antimicrobial efficacies of surface sanitizer solutions. Lauric arginate (LAE) is a cationic surfactant with antimicrobial efficacy against Listeria monocytogenes. In this study, we investigated the antimicrobial and the wettability effects of LAE in enhancing anti-L. monocytogenes efficacy of peracetic acid (PAA) and further verified the optimized treatment combinations in a pilot spray-bar brush bed system. Apples after 48 h of inoculation were treated with PAA surface sanitation in combination with different concentrations of LAE at 22 or 46 degrees C. The effectiveness of PAA with LAE solutions in decontaminating L. monocytogenes significantly increased with the increased concentration of PAA (60-80 ppm) or LAE (0.01-0.05%) or the treatment temperature (from 22 to 46 degrees C). A 30-120-sec wash by 80 ppm PAA with 0.01 and 0.05% LAE at 22 degrees C reduced L. monocytogenes on apples by 2.10-2.25 and 2.48-2.58 log(10) CFU/apple, respectively. Including LAE in the PAA solution decreased contact angles on apple surfaces. However, the increased wettability of the sanitizer solution may not be the main contributor to the enhanced antimicrobial efficacy of the PAA solution, given that the addition of Tween 80 or Tween 85 only slightly boosted the anti-L. monocytogenes efficacy of PAA solutions though both increased the wettability of the PAA solutions. The synergistic effects of PAA and LAE were further validated in a pilot spray-bar brush bed packing system, where a 30-sec spray wash with 80 ppm PAA and 0.05% LAE at 22 and 46 degrees C caused 1.68 and 2.08 log reduction of Listeria on fresh apples, respectively. This study provides an improved PAA process/preventive strategy for ensuring microbial food safety of fresh apples that is applicable to commercial apple packing lines.
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