Contact
    Start Publications Temperature, pH, and Molecular Packing Effects on the ...
    ←  Publication Finder
    KSV NIMA

    Temperature, pH, and Molecular Packing Effects on the Penetration of Oleic Acid Monolayer by alpha-Lactalbumin

    Year: 2019

    Journal: Langmuir, Volume 35, 1-Feb, page 3183–3193

    Authors: Krajewska, Martyna; Dopierala, Katarzyna; Weiss, Marek; Prochaska, Krystyna

    Organizations: National Science Centre, Poland [2017/01/X/ST4/00242]; Ministry of Science and Higher Education in PolandMinistry of Science and Higher Education, Poland [03/32/DSPB/0801]; Ministry of Science and Higher Education in Poland within the project realized at the Faculty of Technical Physics, Poznan University of Technology

    Recently, we reported on the interfacial behavior of mixed oleic acid (OA)-alpha-lactalbumin monolayer and its relevance in the formation of tumoricidal HAMLET (human alpha-lactalbumin made lethal to tumor cells)-like complex. This complex is probably formed in the gastrointestinal tract, but it has not been proved so far. The molecular base and the underlying physicochemical forces leading to such complexation remain to be known as well. There are also several other issues related with the complex stoichiometry that need to be fully explained. This study provides insight into the mechanism of temperature, pH, and physical state of monolayer-dependent binding of OA by the milk protein-apo-alpha-lactalbumin. Using the Langmuir and Langmuir-Blodgett approaches, we investigated the interactions between the OA monolayer and the apo-bovine alpha-lactalbumin (BLA III) at different pH, temperatures, and molecular packing. We found that the most favorable conditions for the formation of mixed OA-BLA III film are relevant to the gastric environment. The stabilization of OA-BLA III at the interface is associated with the conformational changes of protein in the presence of fatty acids induced by low pH and high temperature in the expanded monolayer. Our approach helps to understand the molecular mechanism of HAMLET/bovine alpha-lactalbumin made lethal to tumor cells formation in vivo.
    View publication