Contact
    Start Publications The influence of terpinen-4-ol and eucalyptol - The essential ...
    ←  Publication Finder
    KSV NIMA

    The influence of terpinen-4-ol and eucalyptol - The essential oil components - on fungi and plant sterol monolayers

    Year: 2019

    Journal: Biochim. Biophys. Acta-Biomembr., Volume 1861, 1-Jun, page 1093–1102

    Authors: Polec, Karolina; Wojcik, Aneta; Flasinski, Michal; Wydro, Pawel; Broniatowski, Marcin; Hac-Wydro, Katarzyna

    Keywords: The essential oils; Model membranes; Brewster angle microscopy; GIXD studies

    Antifungal and herbicidal activity of terpenes, being the components of the essential oils, is directly related to the incorporation of these compounds into cellular membranes. Thus, the differences in the lipid composition of various pathogenic membranes may be the factor determining the activity of these molecules. One of the class of lipids, which form the membrane environment are sterols. The aim of this work was to compare the effect of two terpenes: terpinen-4-ol and eucalyptol on the monolayers formed by ergosterol and beta - sitosterol, which are the components of fungi and plant membranes, respectively. The modifications in the sterol monolayer properties were investigated in the surface pressure-area measurements and penetration studies as well as in a micrometer scale (Brewster angle microscopy experiments) and in nanoscale (GIXD technique). It was evidenced that although at higher surface pressure the terpene molecules are in part removed from the interface, they are able to substantially modify the condensation, morphology and molecular organization of the sterol film. It was also found that the incorporation of terpenes into sterol films is comparable for both sterols, however, beta - sitosterol monolayers properties are affected more strongly than ergosterol films. Finally, the analysis of the results of the studies performed on model membrane systems and the results of antimicrobial studies reported in literature, enabled us to suggest that the activity of terpenes depends on the membrane composition and that the sterol concentration may be important from the point of view of antifungal effect of terpinen-4-ol and eucalyptol.
    View publication