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    The shape of lipid molecules affects potential-driven molecular-scale rearrangements in model cell membranes on electrodes

    Year: 2020

    Journal: Bioelectrochemistry, Volume 132, APR

    Authors: Khairalla, B; Juhaniewicz-Debinska, J; Sek, S; Brand, I

    Organizations: DFGGerman Research Foundation (DFG)European Commission [BR-3961-4]; DAADDeutscher Akademischer Austausch Dienst (DAAD)European Commission [57215882]; NCN [UMO-2016/21/B/ST4/02122]

    Keywords: Lipid bilayer; Electrode potential; Membrane potential; Packing parameter; Polarization modulation infrared reflection absorption spectroscopy; Quartz crystal microbalance

    Planar asymmetric lipid bilayers composed of phosphatidylethanolamine and phosphatidylglycerol lipids are transferred onto a gold electrode surface. Lipids containing two saturated, one monounsaturated and two monounsaturated hydrocarbon chains compose the model membranes. Results of electrochemically controlled polarization modulation infrared reflection absorption spectroscopy and quartz crystal microbalance with energy dissipation studies reveal two different types of electric potential dependent structural rearrangements in the bilayers. They are correlated with the geometry of the lipid molecule. Packing parameter correlates the cross-section area of the hydrophobic and hydrophilic parts of amphiphilic molecules. In bilayers composed of lipids with the packing parameter <1, the hydrocarbon chains are tilted with respect to the bilayer plane and the polar head groups are well hydrated. At a threshold potential an abrupt flow of water through the bilayer is connected with membrane dehydration and upward orientation of the chains. In bilayers composed of lipids with packing parameter >= 1, electric potentials have negligible effect on the membrane structure. A simple rule correlating the packing parameter with molecular scale changes occurring at electrified membranes has a large diagnostic implication for biomimetic studies and our understanding of molecular processes occurring in biological cell membranes. (C) 2019 Elsevier B.V. All rights reserved.
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