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    The synthesis of a UCST-type zwitterionic polymer for the efficient recycling of cellulase at room temperature

    Year: 2021

    Journal: Green Chem., Volume 23, APR 7, page 2738–2746

    Authors: Li, Feiyun; Qin, Feiyang; Pang, Yuxia; Lou, Hongming; Cai, Cheng; Liu, Weifeng; Qian, Yong; Qiu, Xueqing

    Organizations: National Natural Science Foundation of China [21978106, 21676109]; National Key RD Plan [2018YFB1501701-1]

    In order to reduce the enzyme cost of lignocellulosic enzymatic hydrolysis, upper critical solution temperature (UCST) additives are used to recover and reuse cellulase by regulating the temperature. A sulfobetaine polymer, PSPE, showed a sensitive UCST response in buffer, and its UCST-performance was optimised by adjusting the molecular weight of the polymer. PSPE-3 (M-w = 355.1 kDa) was added to the cellulase buffer at 50 degrees C, and PSPE-3 and cellulase were co-precipitated by cooling to the room temperature of 25 degrees C. PSPE-3 and cellulase co-precipitated mainly through hydrophobic interactions via cooling. Compared with octadecyl sulfobetaine (SB3-18), PSPE-3 had a more sensitive UCST response and stronger cellulase recovery ability. Adding only 40% PSPE-3 (accounting for the mass fraction of cellulase) can save more than 50% cellulase in the enzymatic hydrolysis system of eucalyptus chips pre-treated with dilute acid (Eu-DA). However, for the Eu-DA system, adding 100% SB3-18 can only save about 30% of cellulase. In this work, efficient recycling of cellulase was achieved at room temperature by adding UCST-type PSPE. The process is green without consuming acid and alkali, and this work provides a new idea for reducing the production cost of cellulosic ethanol.
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