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    Thermodynamics and structure of model bio-membrane of liver lipids in presence of imidazolium-based ionic liquids

    Year: 2021

    Journal: Biochim. Biophys. Acta-Biomembr., Volume 1863, JUN 1

    Authors: Mitra, Saheli; Sharma, Veerendra Kumar; Mitra, Jyotsna Bhatt; Chowdhury, Subhadip; Mukhopadhyay, Mrinmay Kumar; Mukhopadhyay, Ramaprasad; Ghosh, Sajal Kumar

    Organizations: Science & Engineering Research Board (SERB), Department of Science and Technology (DST), India [EMR/2016/006221]; Department of Science and Technology (DST), India, through INSPIRE fellowship

    Keywords: Ionic liquids; Liver lipid; Membrane morphology; Lipid disorder

    Ionic liquids (ILs) are the attractions of researchers today due to their vast area of potential applications. For biomedical uses, it becomes essential to understand their interactions with cellular membrane. Here, the membrane is mimicked with lipid bilayer and monolayer composed of liver lipids extract. Three archetypal imidazolium based ILs, 1-decyl-3-methylimidazolium tetrafluomborate ([DMIM][BF4] or [C10MIM][BF4]), 1-octyl-3-methylimidazolium tetrafluoroborate, ([OMIM][BF4] or [C8MIM][BF4]) and 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIM][B3F4] or [C2MIM][BF4]) having different alkyl chain lengths are used in the present study. The isothermal titration calorimetry (ITC) measurements showed that [DMIM][BF4] interacts strongest with the liver lipid membrane compared to other two ILs which have relatively shorter alkyl chain length. The low values of stoichiometry ratio of ILs indicates that ILs penetrate within the core of the lipid bilayer. The interaction of ILs with the liver lipid membrane is found to be mainly driven by entropy which could be due to the change in the structure of the lipid membrane at local or global scales. Dynamic light scattering (DLS) measurements indicate that there are no changes in the size of vesicles due to addition of [DMIM][BF4] indicating stability of the vesicles. On the other hand, x-ray reflectivity (XRR) measurements showed a concentration dependent change in the monolayer structure. At low concentration of the IL, the monolayer thickness decreases, exhibiting an increase in the electron density of the layer. However, at higher concentrations, the monolayer thickness increases proving a concentration dependent effects of the IL on the arrangement of the molecules.
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