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    Antithrombogenic polysaccharide coatings to improve hemocompatibility, protein-repellence, and endothelial cell response

    Year: 2024

    Authors: Bračič, Matej; Nagy, Bence M.; Plohl, Olivija; Lackner, Florian; Steindorfer, Tobias; Fischer, Roland C.; Heinze, Thomas; Olschewski, Andrea; Kleinschek, Karin Stana; Nagaraj, Chandran; Mohan, Tamilselvan

    Keywords: Biological sciences; Biomaterials; Coatings

    Polyester biomaterials play a crucial in vascular surgery, but suffer from unspecific protein adsorption, thrombogenicity, and inadequate endothelial cell response, which limit their success. To address these issues, we investigated the functionalization of polyester biomaterials with antithrombogenic polysaccharide coatings. A two-step and water-based method was used to coat cationized polycaprolactone with different sulfated polysaccharides (SPS), which resulted in long-term stability, tunable morphology, roughness, film thickness, chemical compositions, zeta potential, and water content. The coatings significantly increased the anticoagulant activity and reduced the thrombogenicity of polycaprolactone, particularly with highly sulfated heparin and cellulose sulfate. Less SPS, such as chondroitin sulfate, fucoidan, and carrageenan, despite showing reduced anticoagulant activity, also exhibited lower fibrinogen adsorption. The adhesion and viability of human primary endothelial cells cultured on modified polycaprolactone correlated with the type and sulfate content of the coatings.
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