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    Highly specific lubricin-lectin electrochemical sensor for glycoprotein cancer biomarker detection

    Year: 2023

    Authors: Li, Miaosi; Abeyrathne, Chathurika; Langley, Daniel P.; Cossins, Luke R.; Samudra, Anushka N.; Green, George W.; Moulton, Simon E.; Silva, Saimon M.

    Keywords: Biosensors; Cancer monitoring; Glycomics analysis; Lectin based biosensors; Lubricin; T antigen; Tn antigen

    Electrochemical glycoprotein sensors have proven to be a great alternative for the detection and characterization of cancer, therapeutic design, and monitoring. A combination of the glycoproteins lubricin (LUB) and peanut agglutinin (PNA) has been successfully implemented for the detection of tumour-associated short glycans directly in bodily fluids, e.g. whole blood. However, such an approach has so far only been studied using a surface-bound redox mediator engineered with the recognition element, PNA. In this paper, we extend the lubricin-peanut agglutinin (LUB-PNA) interface for monitoring glycan binding interactions using redox species in solution. Cyclic voltammetry and electrochemical impedance spectroscopy measurements using the LUB-PNA interface showed that faradaic electron transfer of ferrocyanide redox species in solution is altered in presence of the glycoprotein containing cancer-associated T-antigen glycoprotein (asialofetuin, ASF), while it does not alter in presence of the sialylated glycoform of ASF, fetuin (FET), bovine serum albumin (BSA) and Tn-antigen. The limit of detection (LoD) of ASF is 39 nM in PBS. In addition, this highly specific LUB-PNA sensor allows for the detection of ASF in human blood.
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