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    Acetylene plasma coated surfaces for covalent immobilization of proteins

    Year: 2009

    Journal: Thin Solid Films, 517 (17), 1 July 2009, Pages 5343-5346, 20100827

    Authors: Yin Y. 1, Nosworthy N.J.1, Youssef H. 2, Gong B. 3, Bilek M.M.M. 1, McKenzie D.R. 1

    Last authors: D.R. McKenziea

    Organizations: 1 Applied & Plasma Physics Group, Physics School, University of Sydney, NSW 2006, Australia 2 Mechanical Engineering School, University of Sydney, NSW 2006, Australia 3 Analytic Centre, The University of New South Wales, NSW 2052, Australia

    Country: Australia

    A modified plasma enhanced chemical vapor method was used for acetylene plasma polymerization of biocompatible surfaces on a range of substrates. Smooth polymerized surfaces with excellent mechanical properties were achieved suitable for a wide range of biochemical and biomedical applications. Horseradish peroxidase activity analysis showed that the proteins immobilized on the plasma polymerized surfaces maintained their biological function for a much longer period of time compared to untreated surfaces. The plasma polymerized surfaces and the protein immobilization were also analyzed using quartz crystal microbalance with dissipation analysis, spectroscopic ellipsometry, X-ray photoelectron spectroscopy, and tensile strength analysis. The results indicate that the plasma polymerized surfaces provide covalent bonding sites and immobilize a dense monolayer of proteins after incubation in protein containing solution.