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    Acetylene plasma polymerized surfaces for covalent immobilization of dense bioactive protein monolayers

    Year: 2009

    Journal: Surface and Coatings Technology, 203 (10-11), 25 February 2009, Pages 1310-1316, 20100827

    Authors: Yin Y. 1, Bilek M.M.M 1, McKenzie D.R. 1, Nosworthy N.J. 1, Kondyurin A 1, Youssef H. 2, Byrom M.J. 3, Yang W. 4

    Last authors: Wengrong Yang

    Organizations: 1 Applied and Plasma Physics Group, Physics School, University of Sydney, NSW 2006, Australia 2 Mechanical Engineering School, University of Sydney, NSW 2006, Australia 3 Medicine School, University of Sydney, NSW 2006, Australia 4 Electron Microscope Unit, University of Sydney, NSW 2006, Australia

    Country: Australia

    Smooth polymerized surfaces, suitable for biochemical and biomedical applications, were deposited using a modified plasma enhanced chemical vapour deposition method with acetylene as a reaction precursor. Horseradish peroxidase (HRP) activity assays showed that the protein immobilized on the plasma polymerized surfaces maintained its biological function for a much longer period of time compared to that on uncoated surfaces. The kinetics of HRP attachment to the plasma polymerized surfaces were analyzed using quartz crystal microbalance with dissipation analysis. Spectroscopic ellipsometry and attenuated total reflection Fourier transform infrared spectroscopy were used to determine the thickness and the quantity of the attached protein. The results showed that the plasma polymerized surfaces provided a high density of attachment sites to covalently immobilize a dense monolayer of proteins.