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    Actin Filament Motility Induced Variation of Resonance Frequency and Rigidity of Polymer Surfaces Studied by Quartz Crystal Microbalance

    Year: 2012

    Journal: Langmuir, 2012, 28 (42), pp 15033–15037, 20130116

    Authors: Harm van Zalinge †, Jenny Aveyard †, Joanna Hajne †, Malin Persson ‡, Alf Mansson ‡, Dan V. Nicolau *†

    Last authors: Dan V. Nicolau

    Organizations: † Department of Electrical Engineering and Electronics, University of Liverpool, Brownlow Hill, Liverpool, L69 3GJ, United Kingdom ‡ School of Natural Sciences, Linnaeus University, SE-39245 Kalmar, Sweden

    Country: england, UK, united kingdom, sweden, sverige

    This contribution reports on the quantification of the parameters of the motility assays for actomyosin system using a quartz crystal microbalance (QCM). In particular, we report on the difference in the observed resonance frequency and dissipation of a quartz crystal when actin filaments are stationary as opposed to when they are motile. The changes in QCM measurements were studied for various polymer-coated surfaces functionalized with heavy meromyosin (HMM). The results of the QCM experiments show that the HMM-induced sliding velocity of actin filaments is modulated by a combination of the viscoelastic properties of the polymer layer including the HMM motors.