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    Adhesive nanostructured multilayer films using a bacterial exopolysaccharide for biomedical applications

    Year: 2013

    Journal: J. Mater. Chem. B, 2013,1, 2367-2374, 20130630

    Authors: Rui R. Costa a b, Ana I. Neto a b, Ilker Calgeris c, Clara R. Correia a b, António C. M. Pinho d, Jaime Fonseca e, Ebru T. Öner c, João F. Mano a b

    Last authors: João F. Mano

    Organizations: a 3B's Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas – Guimarães, Portugal E-mail: jmano@dep.uminho.pt ; Fax: +351 253 510 909 ; Tel: +351 253 510 900 b ICVS/3B's, PT Government Associated Laboratory, Braga/Guimarães, Portugal c Marmara University, Department of Bioengineering, Istanbul, Turkey d University of Minho, Department of Mechanical Engineering, Campus de Azurém, Guimarães, Portugal e University of Minho, Department of Industrial Electronics, Campus de Azurém, Guimarães, Portugal

    Country: Portugal

    Medical adhesives and sealants often require that long-term adhesiveness is achieved. In this work, nanostructured coatings consisting of chitosan and the adhesive bacterial exopolysaccharide levan are fabricated using layer-by-layer (LbL) assembly. Taking advantage of the electrostatic self-assembly mechanism of LbL, the charges of both chitosan and a phosphonate-derivatized levan (Ph-levan) are measured and the feasibility of constructing hybrid films is monitored and confirmed using a quartz crystal microbalance with dissipation monitoring (QCM-D). The adhesive properties between two identical bonded films with a total of 100 layers are compared to control films in which Ph-levan is replaced by alginate, revealing that the detachment force of the former is about 3 times higher than the control. Scanning electron microscopy of the films surface shows that the surface of Ph-levan films is smooth and homogeneous. Cell adhesion tests were conducted using a L929 cell line. Early cell adhesion is significantly higher in chitosan/Ph-levan films when compared to chitosan/alginate controls. These findings establish levan derivatives as bioinspired ingredients for conceiving medical adhesive devices that allow achieving enhanced mechanical and biological performance.