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    Adsorption of NGF and BDNF derived peptides on gold surfaces

    Year: 2014

    Journal: Phys. Chem. Chem. Phys., 2014,16, 1536-1544, 20140104

    Authors: Giuseppe Forte 1, Alessio Travaglia 2, Antonio Magrì 3, Cristina Satriano* 4and Diego La Mendola* 5

    Last authors: Diego La Mendola

    Organizations: Phys. Chem. Chem. Phys., 2014,16, 1536-1544 1 Department of Pharmaceutical Sciences, University of Catania, Viale Andrea Doria, 6, Italy 2 Centre for Neural Science, New York University, Washington Place, 4, New York, USA 3 Institute of Biostructures and Bioimages – Catania, National Council of Research (CNR), Viale Andrea Doria, 6, Catania, Italy 4 Department of Chemical Sciences, University of Catania, Viale Andrea Doria, 6, I-95125 Catania, Italy E-mail: csatriano@unict.it; 5 Department of Pharmacy, University of Pisa, via Bonanno Pisano, 6, I-56100 Pisa, Italy

    Country: USA, US, United States, United States of America, America, Italy

    This study tackles the interaction between gold surfaces and two peptide fragments named NGF(1-14) and BDNF(1-12), able to mimic the proliferative activity of the nerve growth factor (NGF) and the brain derived neurotrophic factor (BDNF), respectively. The physical adsorption processes on the solid surface from both single and binary peptide solutions, at physiological and acid pH, were investigated by QCM-D and CD experiments, as well as by molecular dynamics calculations. The relevant physicochemical properties at the hybrid bio-interface, including peptide–surface interaction, conformational changes, hydrodynamic thickness, viscoelastic parameters, and competitive vs. synergic behaviour of the two peptide fragments towards the surface, were scrutinized. Biological assays with neuronal cells pointed to the maintenance of the biological activity of NGF(1-14) and BDNF(1-12) peptide molecules within the adlayers on the gold surface.