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Application of Kevin–Voigt Model in Quantifying Whey Protein Adsorption on Polyethersulfone Using QCM-D

Year: 2009

Journal: Journal of the Association for Laboratory Automation, Volume 14, Issue 4, August 2009, Pages 213-220, 20100827

Authors: Liu S.X. 1, Kim J-T 2

Last authors: Jun-Tae Kim

Organizations: 1National Center for Agricultural Utilization Research, Peoria, IL 2Cornell University, USDA_ARS, Ithaca, NY

Country: USA, US, United States of America

The study of protein adsorption on the membrane surface is of great importance to cheese-making processors that use polymeric membrane-based processes to recover whey protein from the process waste streams. Quartz crystal microbalance with dissipation (QCM-D) is a lab-scale, fast analytical technique that can precisely monitor and quantify the amount of proteins adsorbed onto the polymer surface in real time. The ability of the software that accompanies the instrument to predict the minute amount of the mass of the protein on the surface and the structural properties of the adsorbed protein requires a suitable viscoelastic model to convert the data of frequency and dissipation shift responses generated from the QCM-D apparatus into the mass and viscoelastic properties of the proteins adsorbed on the surface at given times. In this study, data recorded by a QCM-D apparatus were fitted into the Kevin–Voigt model, to quantify the whey protein adsorption on the polyethersulfone membrane surface.