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    Assembly of Poly(dopamine)/Poly(N-isopropylacrylamide) Mixed Films and Their Temperature-Dependent Interaction with Proteins, Liposomes, and Cells

    Year: 2013

    Journal: Langmuir, 2013, 29 (32), pp 10213–10222, 20131001

    Authors: Yan Zhang 1 2, Karthiga Panneerselvam 2, Ryosuke Ogaki 2, Leticia Hosta-Rigau 2, Rebecca van der Westen 2, Bettina E. B. Jensen 3, Boon M. Teo 2, Meifang Zhu *1, and Brigitte Städler *2

    Last authors: Brigitte Städler

    Organizations: 1 State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, Shanghai 201620, People’s Republic of China 2 iNANO, Aarhus University, Gustav Wieds Vej 14, DK-8000 Aarhus, Denmark 3 Department of Chemistry, Aarhus University, Langelandsgade 140, DK-8000 Aarhus, Denmark

    Country: China, Denmark

    Many biomedical applications benefit from responsive polymer coatings. The properties of poly(dopamine) (PDA) films can be affected by codepositing dopamine (DA) with the temperature-responsive polymer poly(N-isopropylacrylamide) (pNiPAAm). We characterize the film assembly at 24 and 39 °C using DA and aminated or carboxylated pNiPAAm by a quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray photoelectron spectroscopy, UV–vis, ellipsometry, and atomic force microscopy. It was found that pNiPAAm with both types of end groups are incorporated into the films. We then identified a temperature-dependent adsorption behavior of proteins and liposomes to these PDA and pNiPAAm containing coatings by QCM-D and optical microscopy. Finally, a difference in myoblast cell response was found when these cells were allowed to adhere to these coatings. Taken together, these fundamental findings considerably broaden the potential biomedical applications of PDA films due to the added temperature responsiveness.