Contact
    Start Publications Bioelectrocatalytic self-assembled thylakoids for micro-power ...
    ←  Publication Finder
    QSense

    Bioelectrocatalytic self-assembled thylakoids for micro-power and sensing applications

    Year: 2006

    Journal: Sensors and Actuators B 117 (2006) 480–487, 20100827

    Authors: Lam K.B. 1 2, Irwin E.F. 3, Healy K.E. 3 4, Lin L. 1 2

    Last authors: Liwei Lin

    Organizations: 1 Department of Mechanical Engineering, University of California at Berkeley, Berkeley, CA 94720, USA 2 Berkeley Sensor and Actuator Center, University of California at Berkeley, Berkeley, CA 94720, USA 3 Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA 4 Department of Materials Science and Engineering, University of California at Berkeley, Berkeley, CA 94720, USA

    Country: USA, US, United States of America

    Photosynthetic sub-cellular plant structures called thylakoid were immobilized onto a gold electrode surface that had been functionalized by bioelectrocatalytic self-assembled monolayers (bio-SAMs) of cystamine and pyrroloquinoline quinone (PQQ). The goal is to achieve direct transfer of electrons from thylakoids to the electrode via the bio-SAMs to increase the electrical efficiency of MEMS photosynthetic electrochemical cells (μPECs). The immobilization technique could also be used in MEMS bio-sensing and microbial fuel cell applications. Quartz crystal microbalance with dissipation (QCM-D) was used to characterize the deposition kinetics of cystamine, PQQ, and thylakoids. Using QCM-D, the surface coverage of these three layers was determined to be, respectively, 7.9 × 10−10 mol/cm2, 3.3 × 10−10 mol/cm2, and 1.5 × 106 thylakoids/cm2. The cystamine and PQQ monolayers formed within 5 min, while the thylakoid layer required over 1 h. Each layer was shown to be covalently linked to the substrate or layer underneath and thus was able to survive repeated rinsing in water or buffer.