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    Biomimetic poly(glycerol sebacate) (PGS) membranes for cardiac patch application

    Year: 2013

    Journal: Materials Science and Engineering, C, Volume 33, Issue 7, October 2013, Pages 3677–3687, 20131119

    Authors: Ranjana Rai, Marwa Tallawi, Niccoletta Barbani, Caterina Frati, Denise Madeddu, Stefano Cavalli,Gallia Graiani, Federico Quaini, Judith A. Roether, Dirk W. Schubert, Elisabetta Rosellini, Aldo R. Boccaccini

    Organizations: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen Nuremberg, 91058 Erlangen, Germany, Department of Chemical Engineering, Industrial Chemistry and Materials Science, Largo Lucio Lazzarino, 56126 Pisa, Italy, Department of Medicine and Pathology, University of Parma, 12-I 43126 Parma, Italy, Institute of Polymeric Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany

    In this study biomimetic poly(glycerol sebacate) PGS matrix was developed for cardiac patch application. Therationale was that such matrices would provide conducive environment for the seeded cells at the interphasewith PGS. From the microstructural standpoint, PGS was fabricated into dense films and porous PGS scaffolds.From the biological aspect, biomimetic PGS membranes were developed via covalently binding peptidesTyr-Ile-Gly-Ser-Arg (YIGSR) and Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP), corresponding to the epitopesequences of laminin and fibronectin, respectively onto the surface. To improve and enhance homogenousbinding of peptides onto the PGS surface, chemical modification of its surface was carried out. A sequentialregime of alkaline hydrolysis with 0.01 M NaOH for 5 min and acidification with 0.01 M HCl for 25 s wasoptimal. More COOH chemical group was exposed without causing deleterious effect on the bulk propertiesof the polymer as revealed by the physicochemical analysis carried out. HPLC analysis, chemical imaging andToF-SIMS were able to establish the successful homogenous functionalization of PGS membranes with thepeptides. Finally, the developed biomimetic membranes supported the adhesion and growth of rat andhuman cardiac progenitor cells.