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    Chemically Detachable Polyelectrolyte Multilayer Platform for Cell Sheet Engineering

    Year: 2012

    Journal: Chem. Mater., 2012, 24 (5), pp 930–937, 20120618

    Authors: Armelle Chassepot†‡, Longcheng Gao§, Isabelle Nguyen†‡, Alexandre Dochter#, Florence Fioretti†‡, Patrick Menu , Halima Kerdjoudj , Corinne Baehr§, Pierre Schaaf#, Jean-Claude Voegel†‡*, Fouzia Boulmedais#, Benoit Frisch§, and Joëlle Ogier†‡*

    Last authors: Joëlle Ogier

    Organizations: † Institut National de la Santé et de la Recherche Médicale, INSERM Unité 977, Faculté de Chirurgie Dentaire, 11 Rue Humann, 67085 Strasbourg Cedex, France ‡ Université de Strasbourg, Faculté de chirurgie dentaire, 1 place de l’Hôpital 67000 Strasbourg § Laboratoire de Conception et Application de Molécules Bioactives, UMR 7199, CNRS/Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch Cedex, France. Group of Bioengineering (UMR CNRS 7561), UHP-Nancy 1, Faculté de Médecine, 9 avenue de la forêt de Haye 54500 Vandoeuvre-lès-Nancy, France Institut National de la Santé et de la Recherche Médicale, INSERM, UMR-S 926, IFR 53 URCA, UFR-Odontologie, 1 avenue du Maréchal Juin, 51095 Reims Cedex, France # Institut Charles Sadron, Université de Strasbourg, Centre National de la recherche scientifique, UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex, France

    Country: France

    Human gingival fibroblasts (HGFs) cell sheets have a potential use for in vivo wound healing due to the ability of HGFs to adopt a contractile phenotype which is typically expressed during extracellular matrix tissue remodeling. For this purpose, we developed a chemically detachable platform based on poly(allylamine hydrochloride)/poly(styrene sulfonate) multilayer film built on a sacrificial precursor film which served as a substrate for HGF cell layer formation. The sacrificial precursor film, based on disulfide-containing polycation and polyanion, is degradable under mild conditions compatible for cell sheet detachment. Cellular viability and cell phenotype analysis of HGF show that the designed platform promotes cell phenotype switch into contractile phenotype, maintained after cell sheet lift-off. This contractile phenotype is acquired by fibroblasts during in vivo wound healing and tissue remodeling. HGFs cell sheet fragments, obtained by this detachment process, could be cultured later on showing a good retention of the typical spindle-shape of differentiated cells after 10 days of culture. HGFs cell sheets have great potential applications as autologous substrates for tissue repair and cellular synthetic platforms for research on connective tissue diseases or evaluation of novel therapeutic agents.