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    Composition and structure of mixed phospholipid supported bilayers formed by POPC and DPPC

    Year: 2012

    Journal: Soft Matter, 2012,8, 5658-5665, 20120618

    Authors: Anna Åkesson 1, Tania Lind 1, Nicky Ehrlich 2, Dimitrios Stamou 2, Hanna Wacklin 3 and Marité Cárdenas 4

    Last authors: Marité Cárdenas

    Organizations: 1. Institute of Chemistry and Nano-Science Center,University of Copenhagen,Universitetsparken 5, Copenhagen, Denmark 2. Bio-Nanotechnology Laboratory,Department of Neuroscience Pharmacology,Nano-Science Center and Lundbeck Foundation Center for Biomembranes in Nanomedicine,University of Copenhagen,Universitetsparken 5, Copenhagen, Denmark 3. European Spallation Source, Lund, Sweden 4. Institute of Chemistry and Nano-Science Center,University of Copenhagen,Universitetsparken 5, Copenhagen, Denmark

    Country: Sverige, Sweden, Denmark

    In this paper we present a systematic study of the morphology and composition of supported lipid bilayers (SLBs) formed by vesicle fusion using a wide variety of surface sensitive techniques that give information about the lateral as well as vertical structure and bilayer fluidity. SLBs of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) mixtures at five different bulk vesicle compositions were formed in such a way that the phase separation boundaries were crossed. For all compositions studied, the SLBs were systematically enriched with POPC compared to the nominal vesicle composition. Nevertheless, gel-fluid domain coexistence was observed for SLB compositions in which phase separation was expected based on the bulk phase diagram. The probable causes for the compositional difference in the SLBs are discussed in terms of the phase behaviour of the mixture and its effect on the membrane formation process by vesicle fusion.