Contact
    Start Publications Conformational Changes of Fibrinogen after Adsorption
    ←  Publication Finder
    Attension

    Conformational Changes of Fibrinogen after Adsorption

    Year: 2005

    Journal: J. Phys. Chem. B 109 (2005) 22027-22035, 20111221

    Authors: Matthew L. Clarke, Jie Wang, and Zhan Chen

    Organizations: Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109

    The adsorption behavior of fibrinogen to two biomedical polyurethanes and a perfluorinated polymer has been investigated. Changes in the secondary structure of adsorbed fibrinogen were monitored using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and sum frequency generation vibrational spectroscopy (SFG). SFG measurements were performed in the amide I range as well as in the C-H/N-H stretching range. Amide I signals from SFG demonstrate that fibrinogen has post-adsorption conformational changes that are dependent upon the polymer surface properties. For example, strong attenuation of the amide I and N-H stretching signals with increasing residence time was observed for fibrinogen adsorbed to poly- (ether urethane) but not for the other two polymers. This change is not readily observed by ATR-FTIR. Differences in the observed spectral changes for fibrinogen adsorbed to each polymer are explained by different initial binding mechanisms and post-adsorption conformational changes.